论文部分内容阅读
目的 探讨结构型一氧化氮合酶 (cNOS)mRNA表达在新生鼠缺氧性脑损伤发病中的作用。方法 取新生SD大鼠 2 5只随机分组制作模型并行病理鉴定 ;采用RT -PCR测定脑缺氧 1h ,4h和对照组脑组织细胞内cNOSmRNA表达的变化 ,辉度扫描获取数值。结果 鼠缺氧 1h后脑组织中cNOSmRNA表达显著上升 (P <0 .0 1 ) ,4h组较 1h组下降 (P <0 .0 1 ) ,但仍显著高于对照组 (P <0 .0 1 )。结论 脑缺氧早期cNOSmRNA表达增加 ,由其合成的NO对受损脑组织可能起保护作用。
Objective To investigate the role of structural nitric oxide synthase (cNOS) mRNA expression in the pathogenesis of hypoxic brain injury in neonatal rats. Methods Twenty-five newborn SD rats were randomly divided into three groups. The changes of cNOS mRNA expression in brain tissue were detected by RT-PCR at 1 h, 4 h after hypoxia and the control group. Results The expression of cNOSmRNA in brain was significantly increased 1 h after hypoxia (P <0.01), but decreased in 4h group compared with 1h group (P <0.01), but still significantly higher than that in control group (P <0.01) ). Conclusions The expression of cNOSmRNA increases in the early stage of cerebral hypoxia, and the synthesis of nitric oxide may play a protective role in the damaged brain.