MIMO has become a core technology of 5G network to largely improve system throughput. Due to the cost and size of the user equipment(UE), the application of MIMO uplink is limited by the difficulty in practical implementation at the user side. Virtual MIMO has been widely investigated to solve this problem for wireless uplink systems. However, virtual MIMO transmission leads to performance degradation due to the multiuser interference. To obtain good trade-off between the system throughput and transmission performance,we investigate joint user grouping and resource allocation under the consideration of system throughput and average mean squared error(MSE) performance in SC-FDMA uplink systems. Based on linear MIMO detection,we first develop MSE-oriented user grouping criteria for evaluation of transmission performance, then establish dynamic user grouping and optimal resource allocation problems for hard and elastic average MSE constraints.The proposed joint resource allocation algorithm is evaluated in SC-FDMA uplink scenarios and the results show that it achieves maximum system throughput with average MSE guaranteed for the hard MSE constraint algorithms and the alterable trade-off between system throughput and average MSE for the elastic MSE constraint algorithms. MIMO has become a core technology of 5G network to substantially improve system throughput. Due to the cost and size of the user equipment (UE), the application of MIMO uplink is limited by the difficulty in practical implementation at the user side. However, virtual MIMO transmission leads to performance degradation due to the multiuser interference. To obtain good trade-off between the system throughput and transmission performance, we investigate joint user grouping and resource allocation under based on linear MIMO detection, we first develop MSE-oriented user grouping criteria for evaluation of transmission performance, then establish dynamic user grouping and optimal resource (MSE) performance in SC-FDMA uplink systems. allocation problems for hard and elastic average MSE constraints.The proposed joint resource allocation algorit hm is evaluated in SC-FDMA uplink scenarios and the results show that it achieves maximum system throughput with average MSE guaranteed for the hard MSE constraint algorithms and the alterable trade-off between system throughput and average MSE for the elastic MSE constraint algorithms.