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为比较正常妊娠、先兆子痫和子痫产妇胎盘绒毛匀浆中一氧化氮合酶(NOS)活性和总亚硝酸盐含量进行研究。31例晚期妊娠伴先兆子痫(轻、重度)及子痫患者和32例正常晚期妊娠孕妇为观察对象,均在产后立即从胎盘子叶下部1/3处取两块大小为1cm×1cm×1cm的胎盘组织,盐水清洗,-70％冰冻切片,在缓冲液中制成有绒毛的匀浆。匀浆4℃低温离心20min,所得上清液用于分析。一氧化氮(NO)的氧化产物有硝酸盐和亚硝酸盐,将硝酸盐还原为亚硝酸盐,采用Griess反应测定总亚硝酸盐的浓度,从而确定NO的产量。利用NO可将氧合血红蛋白氧化为正铁血红蛋白这一性质,用分光光
In order to compare the activities of nitric oxide synthase (NOS) and total nitrite in placenta homogenate of normal pregnancy, preeclampsia and eclampsia, 31 cases of late pregnancy with preeclampsia (mild and severe) and eclampsia and 32 cases of normal late pregnancy pregnant women were observed immediately after birth from the placental cotyledons 1/3 take two pieces of size 1cm × 1cm × 1cm Placenta tissue, saline wash, -70% frozen section, and villous homogenate in buffer. Homogenate at 4 ℃ low temperature centrifugation 20min, the resulting supernatant for analysis. Nitric oxide and nitrite were the oxidation products of nitric oxide (NO). The nitrate was reduced to nitrite. The total nitrite concentration was determined by Griess reaction to determine the production of NO. The use of NO can oxidize oxyhemoglobin to methemoglobin, a property that is spectrally resolved