在接种箱里接种,一般是用酒精灯消毒接种针和封口来防污染,但使用酒精灯有以下缺点。(1)调整火焰大小必须熄灯后方能扯动灯芯;(2)由于接种箱密闭长时间使用会因缺氧而熄灯。必须开箱换气,熏蒸灭菌后才能再用,这样会增加污染;(3)操作时稍不注意会碰倒酒精灯而引起着火;(4)酒精烧完后要熄灯添加酒精。笔者采用沼气灯头改制成沼气接种灯,疑多次试用效果良好,现简介如下:在接种箱底板中央开一个直径1.5厘米孔,把沼气灯引射管套上螺丝和橡皮垫圈后,从上向下穿入孔 Vaccination in vaccination box, usually with alcohol lamp disinfection vaccination needle and seal to prevent pollution, but the use of alcohol lamp has the following disadvantages. (1) adjust the size of the flame must be turned off after the lamp can pull the wick; (2) due to vaccination box closed for a long time due to lack of oxygen and lights. Must be out of the box ventilation, fumigation sterilization before reuse, it will increase the pollution; (3) the operation is not careful will knock down the alcohol lamp and cause a fire; (4) after the alcohol burned to add light alcohol. The author uses biogas lamp to transform into biogas inoculation lamp, suspected trial results are good, are as follows: Inoculation box to open a central hole diameter of 1.5 cm hole, the biogas lamp lead tube set screw and rubber gasket, from the upstream Under the hole into