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目的 在国内前人建立动物破骨细胞骨髓培养方法的基础上，首次建立人破骨细胞骨髓培养法。方法 采取１３例２５－７５岁行全髋置换术的成人股骨上端红骨髓，经密度梯度离心得骨髓单个核细胞（ｍａｒｒｏｗｍｏｎｏｎｕｃｌｅａｒｃｅｌｌｓ，ＭＭＮＣ），在浓度为１ｘ１０－８ｍｏｌ／Ｌ的１，２５－（ＯＨ）２Ｄ３的促分化作用下，于预置盖玻片或人胫骨皮质骨片的２４孔培养板内，每孔接种１ｘ１０６／ｍｌ培养１－３周。结果光镜下观察有单个核细胞融合成多核细胞和单个核细胞团形成，经ＴＲＡＰ染色呈阳性反应，电镜下观察有典型的骨吸收陷窝形成。结论 这些均表明由骨髓培养破骨细胞样细胞的方法是成功的。而且利用此方法较之新生动物的直接分离法，所得破骨细胞量多，特征明显，并且方法简单易行。更重要的是为人的破骨细胞样细胞，因此培养方法更有价值。
OBJECTIVE: To establish the human osteoclast bone marrow culture method for the first time on the basis of establishing animal osteoclast bone marrow culture methods in China. Methods Thirteen patients with 25-75 year-old total hip arthroplasty were divided into 3 groups: the upper femur bone marrow of the femur and the marrow mononuclear cells (MMNC) by density gradient centrifugation. ) 2D3, in a 24-well culture plate with preset coverslips or human tibia cortical bone slices, and inoculate 1 × 10 6 / ml for 1 to 3 weeks in each well. Results The mononuclear cells were fused to form mononuclear cells and mononuclear cell clusters under light microscope. The positive cells were detected by TRAP staining and the typical bone resorption lacuna was observed under electron microscope. Conclusions These indicate that the method of culturing osteoclast-like cells from bone marrow is successful. And the use of this method than the newborn animal’s direct separation method, the resulting amount of osteoclasts, characteristics are obvious, and the method is simple and easy. More important is the human osteoclast-like cells, so the culture method is more valuable.