基于精子顶体酶的溶蛋白特性,我们建立了一种简便的检测精子顶体酶活性的方法。此法以固定明胶薄膜为底物来显示精子顶体酶的活性。本实验用此法测定了三种哺乳类动物精子的顶体酶,结果表明,该法简便、可靠,并且可连续观察单个顶体酶的活性反应,很适合于用作临床男性不孕症检查的常规方法。 Based on the sperm acrosin-like proteolytic properties, we established a simple and convenient method for detecting sperm acrosin activity. This method uses a fixed gelatin film as a substrate to show sperm acrosin activity. This experiment was used to determine the sperm enzymes of three kinds of mammalian sperm, the results show that the method is simple, reliable, and continuous observation of the activity of a single acrosin activity, it is suitable for clinical male infertility check The conventional method.