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通过厌氧批实验的方法,探讨了在硫酸盐还原菌(SRB)法处理模拟酸性矿山排水(AMD)的过程中,以油菜秸秆为碳源时,Zn2+浓度对SRB活性的影响.结果表明,在60 d实验中,以油菜秸秆为碳源时,当Zn2+初始浓度在73.7～196.8 mg.L-1范围时,SRB具有良好活性,实验结束时,pH从初始的5.0上升至中性范围,硫酸根还原率达到96%以上,同时Zn2+浓度降至0.05 mg.L-1以下.Tessier固体形态分类、场发射扫描电镜(FE-SEM)和X射线衍射仪(XRD)分析发现,Zn以有机物及硫化物的形态被固定,其中硫化物主要为闪锌矿(ZnS).当Zn2+初始浓度为262.97 mg.L-1时,SRB的活性受到强烈的抑制,实验结束时,pH从初始的5.0降至4.0左右,硫酸根还原率只有27%,Zn2+维持在较高浓度范围(25 mg.L-1).油菜秸秆可以作为SRB法长期处理AMD的缓释碳源,能为微生物生长繁殖提供物质和能量;秸秆的吸附性可降低Zn2+的生物毒性,使得SRB可以适应高浓度的Zn2+;SRB可以通过形成硫化物矿物的形式固定元素Zn.
The effects of Zn2 + concentration on the SRB activity were studied in anaerobic batch experiments using rape straw as carbon source in SRB treatment of simulated acid mine drainage (AMD) In the 60 d experiment, when rape straw was used as carbon source, SRB had a good activity when the initial concentration of Zn2 + was in the range of 73.7-196.8 mg.L-1. At the end of the experiment, the pH increased from 5.0 to the neutral range, The sulfate radical reduction rate reached more than 96% and the concentration of Zn2 + decreased below 0.05 mg.L-1.Tessier solid state classification, field emission scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) And sulfide were immobilized, of which the sulfide was mainly sphalerite (ZnS). The activity of SRB was strongly inhibited when the initial concentration of Zn2 + was 262.97 mg.L-1. At the end of the experiment, the pH ranged from 5.0 To about 4.0, the sulfate reduction rate was only 27% and the Zn2 + concentration was maintained at a higher concentration range (25 mg.L-1) .Rapeseed straw could be a long-term treatment of the sustained-release carbon source of AMD by SRB method, which could provide the microbial growth and reproduction Material and energy; straw adsorption can reduce the biological toxicity of Zn2 +, making SRB can adapt to high concentrations of Zn 2+; SRB can fix element Zn by forming sulfide minerals.