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目的探讨苦瓜提取物对小鼠免疫功能的影响。方法 200只雌性BALB/c小鼠,以体重分层随机分为4个大组,每大组50只,每大组再按体重随机分为5组,每组10只,分别为:阴性对照组(纯净水)、阳性对照组(环磷酰胺)、苦瓜水提物低、中、高剂量组(1.11、3.33、10.00 g/kg BW),连续灌胃30 d,观察苦瓜提取物对小鼠体液免疫、细胞免疫和非特异性免疫的影响。结果苦瓜提取物中、高剂量组IgA水平较阴性对照组升高(P<0.05),苦瓜提取物中剂量组IgG水平较对照组升高(P<0.05),苦瓜提取物低剂量组IL-6、IL-4水平较阴性对照组升高(P<0.05)。其他指标较对照组差异无统计学意义(P>0.05)。结论在本研究剂量下,苦瓜提取物对正常BALB/c小鼠免疫功能有一定的调节作用,尤其对体液免疫及细胞免疫可能有促进的作用,对非特异性免疫无明显影响。
Objective To investigate the effect of bitter melon extract on immune function in mice. Methods 200 female BALB / c mice were randomly divided into four groups according to their body weight. Each group consisted of 50 rats. Each group was divided into 5 groups according to body weight randomly, 10 rats in each group: negative control Group (pure water), positive control group (cyclophosphamide), bitter water extract low, middle and high dose group (1.11,3.33,10.00 g / kg BW), continuous gavage for 30 days, Murine humoral immunity, cellular immunity and non-specific immune effects. Results Compared with the control group, the level of IgA in middle and high dose group increased significantly (P <0.05), the level of IgG in middle dose group increased (P <0.05), the level of IL- 6, IL-4 levels than the negative control group increased (P <0.05). Other indicators than the control group, no significant difference (P> 0.05). Conclusion The extract of Momordica charantia could regulate the immune function of normal BALB / c mice at the dose of this study, especially for humoral and cellular immunity, and had no significant effect on non-specific immunity.