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目的:观察羟基红花黄色素A(HSYA)对激素性股骨头缺血坏死兔股骨头C-Jun氨基末端激酶1(JNK1)表达的影响。方法:48只新西兰兔随机分为空白组(12只)和模型组(36只)。臀肌注射醋酸泼尼松龙建立股骨头缺血坏死模型。6周后光、电镜观察股骨头。造模成功后剩余模兔随机分为模型对照组、髓芯减压组、生理盐水+髓芯减压组、HSYA+髓芯减压组,髓芯减压组行髓芯减压术,生理盐水+髓芯减压组、HSYA+髓芯减压组分别行髓芯减压术配合髓腔内注射0.9%氯化钠溶液或HSYA,余组不处理。1周后RT-PCR技术检测兔股骨头JNK1 m RNA表达,免疫组化法检测其蛋白表达。结果:1光、电镜观察:模型组空缺骨陷窝明显增多,髓腔脂肪细胞增多,异染色质部分边聚,与空白组比较,差异显著(P<0.05)。2JNK1表达:与模型对照组比较,其余4组JNK1 m RNA及蛋白表达均显著降低(P<0.05);与髓芯减压组比较,HSYA+髓芯减压组两者表达明显降低(P<0.05)。结论:HSYA通过降低细胞凋亡相关的JNK1表达,抑制股骨头髓腔内细胞凋亡,对抗激素所致股骨头缺血坏死的损伤。
Objective: To observe the effect of hydroxysafflor yellow A (HSYA) on the expression of C-Jun N-terminal kinase 1 (JNK1) in the femoral head of rabbits with steroid-induced avascular necrosis of the femoral head. Methods: 48 New Zealand white rabbits were randomly divided into blank group (12 rabbits) and model group (36 rabbits). Gluteal injection of prednisolone acetate model of avascular necrosis of the femoral head. After 6 weeks, the femur head was observed by electron microscope. The remaining model rabbits were randomly divided into model control group, core decompression group, saline + core decompression group, HSYA + core decompression group, core decompression group core decompression, saline + Core decompression group, HSYA + core decompression group respectively core decompression with intramedullary injection of 0.9% sodium chloride solution or HSYA, the remaining group not treated. One week later, the expression of JNK1 mRNA in rabbit femoral head was detected by RT-PCR and the protein expression was detected by immunohistochemistry. Results: (1) Light and electron microscopy showed that in the model group, the number of lacunae increased obviously, the number of adipocytes in the medullary cavity increased, and the heterochromatin was partially aggregated. Compared with the blank group, the difference was significant (P <0.05). Compared with the model control group, the expression of JNK1 m RNA and protein in the other four groups were significantly decreased (P <0.05). Compared with the core decompression group, the expression of JNK1 mRNA and protein in the four groups were significantly decreased (P <0.05 ). CONCLUSION: HSYA can prevent the injury of femoral head necrosis caused by hormones by decreasing the expression of JNK1 related to apoptosis, inhibiting the apoptosis in femoral head marrow cavity.