近年来关于细胞色素P450(CYP450)及其同工酶在药物代谢、毒理学、肿瘤生化、临床合理用药和新药研发等方面的研究都取得了很大进展。“Cocktail”探针药物法作为一种快速、有效的评价药物对CYP450酶活性影响的方法,有着不可替代的优势和广阔的应用前景。为此文章通过总结归纳近年来国内外的相关文献,介绍了“Cocktail”探针药物法的概念、优势和基本研究思路,并对其方法学的验证与优化,探讨药物间的相互作用,评价肝、肾功能,优化给药剂量等方面进行了综述。 In recent years, great progress has been made in the researches on cytochrome P450 (CYP450) and its isoenzymes in drug metabolism, toxicology, tumor biochemistry, clinical rational drug use and new drug research and development. “Cocktail ” probe drug method as a rapid and effective method to evaluate the effect of drugs on CYP450 enzyme activity has irreplaceable advantages and broad application prospects. This article summarizes the relevant literature at home and abroad in recent years, introduces the concept, advantages and basic research ideas of “Cocktail ” probe drug method, and verifies and optimizes its methodology to explore the interaction between drugs , Evaluation of liver and kidney function, optimize the dosage and other aspects were reviewed.