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我们采用单细胞凝胶电泳新技术和ＳＣＥ试验研究了８３－１除草剂的主要代谢产物２，４－二氯－６－胺基酚（ＤＣＡＰ）对Ｖ７９细胞的ＤＮＡ损伤作用。结果表明，ＤＣＡＰ浓度≥５０μｇ／ｍｌ时，细胞出现明显的ＤＮＡ迁移和姐妹染色单体交换（Ｐ＜０．０１），说明ＤＣＡＰ是ＤＮＡ损伤剂。结合前期研究结果推断，６位硝基还原成胺基是该除草剂在体内活化致癌的基本途径。结果还表明，单细胞凝胶电泳比ＳＣＥ试验更灵敏，可能是研究环境低剂量暴露的遗传危害最有效的手段。
We used single-cell gel electrophoresis (SCE) and SCE to study DNA damage of V79 cells induced by 2,4-dichloro-6-aminophenol (DCAP), the main metabolite of 83-1 herbicide. The results showed that when the concentration of DCAP ≥ 50μg / ml, the cells showed significant DNA migration and sister chromatid exchange (P <0.01), indicating that DCAP is DNA damage agent. Combined with the results of the previous study concluded that the 6-nitro reduction to amine is the basic way of activating the herbicide in vivo. The results also show that single cell gel electrophoresis is more sensitive than the SCE assay and may be the most effective means of studying the genetic hazards of low-dose exposure to the environment.