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抗精子抗体明显的存在于人群中的不孕者。用单克隆抗体测定精子成分的特性,可以使人领悟到与人抗精子抗体反应的抗原的性质。作者用凝集、免疫荧光、酶联免疫测定法(ELISA)和淘洗法测定比较一些针对人精子的Mabs。作者观察到当用不同方法测定时,不同Mabs的抗精子活性间存在很大差别。人精子冷冻贮存在卵黄囊稀释液中,用于测定抗精子活性。用人工阴道法得到兔精子。将小鼠副睾研碎加入磷酸盐缓冲液,吸出上清,得到小鼠精子。人精子免疫BALB/C小鼠脾细胞与P3×63Ag8653骨髓瘤细胞融合。经有限稀释克隆化1～2次用1%琼脂进行免疫扩散,以类特异性抗血清测定
Anti-sperm antibodies are clearly present in the population of infertile people. Monoclonal antibodies can be used to determine the characteristics of the sperm component to reveal the nature of the antigen that reacts with human anti-sperm antibodies. The authors compared some of the Mabs to human sperm by agglutination, immunofluorescence, enzyme-linked immunosorbent assay (ELISA) and panning assays. The authors observed that when tested by different methods, there is a big difference between the antisperm activities of different Mabs. Human sperm were cryopreserved in a yolk sac diluent and used to determine antisperm activity. Rabbit sperm with artificial vaginal method. Mouse epididymis was added to the phosphate buffer, the supernatant was aspirated to obtain mouse sperm. Human sperm immunized BALB / c mouse spleen cells were fused with P3 × 63Ag8653 myeloma cells. After limited dilution cloned 1 or 2 times with 1% agar immunodiffusion, class-specific antiserum assay