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目的分析肝穿刺术后BALB/c小鼠肝脏基因表达谱的变化。方法10只BALB/c随机分为两组;实验组和正常组,每组各5只;实验组小鼠开腹直视下肝左叶注生理盐水,8天后采集肝组织标本并提取RNA,cDNA微阵列分析25 000个基因在实验组和正常组小鼠肝组织中的表达水平差异。结果肝穿刺术后共有82条差异基因,其中52条基因表达上调,30条基因表达下调;功能聚类可分为代谢、转录调节、转运、信号转导、炎症反应、细胞增殖与凋亡、细胞周期与细胞骨架及细胞粘附等8类。结论肝穿刺术所致小鼠肝脏基因表达谱发生的改变,涉及多个基因表达调控途径。
Objective To analyze the changes of liver gene expression profiles in BALB / c mice after hepatic puncture. Methods Ten BALB / c mice were randomly divided into two groups: experimental group and normal group, each group had 5 mice in each group. The rats in the experimental group were injected normal saline under the left hepatic lobe under open vision and 8 days later, cDNA microarray analysis of 25 000 genes in the experimental group and normal mice liver tissue expression level differences. Results There were 82 differential genes after hepatic puncture, of which 52 genes were up-regulated and 30 genes were down-regulated. Functional clustering could be divided into metabolic, transcriptional regulation, transport, signal transduction, inflammatory response, cell proliferation and apoptosis, Cell cycle and cytoskeleton and cell adhesion and other 8 categories. Conclusion Liver gene expression changes induced by liver biopsy involve multiple gene expression and regulation pathways.