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丹酚酸A(salvianolic acid A,Sal A)是从丹参根中分离得到的具有多种药理活性的化合物。本文建立的恒河猴血浆中丹酚酸A的LC-MS测定方法可用于研究丹酚酸A在恒河猴体内的药代动力学特性,并考察了同时测定猴血浆中丹酚酸A、B和C的可行性。恒河猴静脉注射丹酚酸A 2.5、5和10 mg·kg-1后,2 min时的血药浓度(C2 min)分别为(28.343±6.426)、(45.679±12.301)和(113.293±24.360)mg·L-1。药时曲线下面积(AUC0–∞)分别为(3.316±0.871)、(5.754±2.150)和(13.761±2.825)μg·L-1·h。本研究为丹酚酸A的临床前及临床研究提供了数据支持,也为丹酚酸A、B和C在猴或人体内的测定提供了参考。
Salvianolic acid A (Sal A) is a compound isolated from Salvia root with a variety of pharmacological activities. The LC-MS method for the determination of salvianolic acid A in rhesus monkey established in this paper can be used to study the pharmacokinetics of salvianolic acid A in rhesus monkeys and to study the simultaneous determination of salvianolic acid A, B and C feasibility. The plasma concentration (C2 min) at 2 min after intravenous salvianolic acid A 2.5, 5 and 10 mg · kg-1 were (28.343 ± 6.426), (45.679 ± 12.301) and (113.293 ± 24.360 ) mg · L-1. The area under the curve (AUC0-∞) was (3.316 ± 0.871), (5.754 ± 2.150) and (13.761 ± 2.825) μg · L-1 · h, respectively. This study provides data support for the preclinical and clinical studies of salvianolic acid A and also provides a reference for the determination of salvianolic acid A, B and C in monkeys or humans.