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目的体外观察中药木香、黄精提取液抑制痢疾杆菌氯霉素耐药株(S.flexneRiD15 R-plasmid CmR)和大肠埃希菌氯霉素敏感株(E.coli1485 RifR)间的R质粒接合传递作用。方法木香、黄精提取液分别与S.flexneRiD15 R-plasmid CmR和E.coli1485 RifR共同培养24 h和48 h后接种于麦康凯琼脂平板,37℃培养24 h,计算木香、黄精及不加药对照组的R质粒转移率和R质粒转移抑制率。结果24 h R质粒转移率木香组为(4.60±0.14)%,黄精组为(4.15±0.78)%,未加药对照组为(47.3±0.53)%,差异有统计学意义(P<0.01);48 h R质粒转移率木香组为(3.65±0.07)%,黄精组为(2.8±0.71)%,未加药对照组为(68.90±1.01)%,差异有统计学意义(P<0.01)。24 h的R质粒转移抑制率木香组为(90.30±8.33)%,黄精组为(91.22±7.76)%;48 h的R质粒转移抑制率木香组为(94.70±9.68)%,黄精组为(95.90±10.23)%。结论木香、黄精均具有体外抑制S.flexneRiD15 R-plasmid CmR和E.coli1485 RifR间R质粒接合传递的作用。
OBJECTIVE To observe the inhibitory effect of traditional Chinese medicine MuXiang and Polygonatum spp. R plasmids on the delivery of S.flexneRiD15R-plasmid CmR and E.coli1485RifR in vitro, effect. Methods The extract of Polygonatum sibiricum and Polygonatum sibiricum was co-cultured with S.flexneRiD15 R-plasmid CmR and E.coli1485 RifR respectively for 24 h and 48 h and then inoculated on MacConkey agar plate and cultured at 37 ℃ for 24 h. Drug control group R plasmid transfer rate and R plasmid transfer inhibition rate. Results The rate of plasmid transfer in the 24 h R group was (4.60 ± 0.14)% in the woody group and (4.15 ± 0.78)% in the Huangjing group and (47.3 ± 0.53)% in the untreated group, with significant difference (P <0.01 ). The rate of plasmid transfer in the 48 h R group was (3.65 ± 0.07)% in the woody group and (2.8 ± 0.71)% in the Huangjing group and (68.90 ± 1.01)% in the unadded control group, with significant difference (P < 0.01). The inhibitory rate of R plasmid transfer in the 24 h group was (90.30 ± 8.33)% in the woody group and (91.22 ± 7.76)% in the Huangjing group, (94.70 ± 9.68)% in the 48 h group (95.90 ± 10.23)%. CONCLUSION MuXiang and Polygonatum all have the effect of inhibiting the R-plasmid junctional transmission between S.flexneRiD15R-plasmid CmR and E.coli1485RifR in vitro.