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探讨过氧化物诱导人类肝细胞（ＬＯ２细胞）、内皮细胞（ＥＶＣ３０４细胞）凋亡时Ｃａ２＋流变化不同特点和意义及丹参提取物Ｒｘａ通过Ｃａ２＋信号系统介导的细胞保护效应。方法用膜联蛋白荧光素（Ａｎｎｅｘｉｎ－Ｖ－Ｆｌｕｏｓ）与碘化丙啶（ＰＩ）双标记流式细胞术（ＦＣＭ）分析Ｈ２Ｏ２作用不同时限Ｒｘａ处理组与未处理组凋亡细胞指数分布；用全细胞膜片钳放大系统显微荧光检测术同步分析同期Ｃａ２＋流变化。结果Ｈ２Ｏ２诱导细胞凋亡时ＬＯ２细胞［Ｃａ２＋］ｉ、Ｃａ２＋跃升，Ａｎｎｅｘｉｎ－Ｖ＋细胞指数均较ＥＶＣ３０４细胞为高；［Ｃａ２＋」ｉ＞４００ｎｍｏｌ／Ｌ后１～２小时可观察到细胞凋亡早期膜翻转现象。Ｒｘａ组［Ｃａ２＋］ｉ、Ａｎｎｅｘｉｎ．Ｖ＋和ＰＩ＋细胞指数均较未处理组为低。结论Ｈ２Ｏ２可诱导ＬＯ２、ＥＶＣ３０４细胞Ｃａ２＋依赖性细胞凋亡；［Ｃａ２＋］ｉ＞４００ｎｍｏｌ／Ｌ可能为细胞凋亡启动的早期信号；ＬＯ２细胞较ＥＶＣ３０４细胞抗凋亡能力为差；Ｒｘａ能有效降低［Ｃａ２＋］ｉ，减轻细胞损伤程度。
To investigate the different characteristics and significance of Ca2 + flux induced by peroxides in apoptosis of human hepatocytes (LO2 cells) and endothelial cells (EVC304 cells) and the cytoprotective effect of Salvia miltiorrhiza extract Rxa mediated by Ca2 + signaling system. Methods The exponential distribution of apoptotic cells in Rxa-treated and untreated groups was analyzed by Annexin-V-Fluos and Propidium Iodide (PI) double labeling flow cytometry (FCM) Whole Cell Patch Clamp Magnification System Simultaneous Analysis of Ca2 + Fluctuation by Microfluorescence Assay. Results Compared with EVC304 cells, [Ca2 +] i and Ca2 + up-regulated and [Ca2 +] i and Ca2 + up-regulated in H2O2-induced apoptosis were both higher than those in EVC304 cells; early apoptotic cells were observed at 1-2 hours after [Ca2 +] i> 400nmol / Flip phenomenon. Rxa group [Ca2 +] i, Annexin. V + and PI + cell index were lower than untreated group. Conclusion H2O2 can induce Ca2 + -dependent cell apoptosis in LO2 and EVC304 cells. [Ca2 +] i> 400nmol / L may be an early signal of apoptosis initiation. LO2 cells have poorer anti-apoptotic ability than EVC304 cells. Ca2 +] i, reduce the degree of cell damage.