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目的检测PTEN和Ki-67在甲状腺癌组织中的表达及其临床意义。方法收集甘肃省天水市第一人民医院2012年9月至2015年3月期间手术切除后经病理证实为原发性甲状腺癌的石蜡包埋组织40例及术后离体组织14例,分别应用免疫组织化学SP法和RT-PCR法检测PTEN和Ki-67蛋白和m RNA在甲状腺癌组织及其相应癌旁组织中的表达,并分析PTEN和Ki-67蛋白表达与甲状腺癌患者临床病理指标之间的关系。结果1 PTEN蛋白表达阳性率在甲状腺癌组织中明显低于其在相应癌旁组织中的表达阳性率〔35.0%(14/40)比60.0%(24/40),P<0.05〕,Ki-67蛋白表达阳性率在甲状腺癌组织中明显高于其在相应癌旁组织中的表达阳性率〔72.5%(29/40)比42.5%(17/40),P<0.05〕。2 PTEN m RNA表达相对灰度值在14例甲状腺癌标本中明显低于其在相应癌旁组织中的表达(0.225 7±0.036 3比0.503 6±0.037 5,P<0.05);Ki-67 m RNA表达相对灰度值在14例甲状腺癌标本中明显高于其在相应癌旁组织中的表达(1.212 1±0.042 1比0.293 6±0.027 4,P<0.05)。3 PTEN和Ki-67蛋白表达与甲状腺癌的组织学分级、病理类型、肿瘤分期及有无区域淋巴结转移有关(P<0.05),而与甲状腺癌患者的性别、年龄及肿瘤包膜是否完整无关(P>0.05)。4 PTEN蛋白和Ki-67蛋白在甲状腺癌中的表达呈明显负相关(rs=-0.605,P=0.000),而其在相应癌旁组织中的表达无相关性(rs=-0.021,P=0.899)。结论 PTEN和Ki-67基因在甲状腺癌组织中异常表达,其可能与甲状腺癌的发生、发展及其机制有一定关系,二者联合可能有助于甲状腺癌病理类型的鉴别、生物学行为的判断和临床分期,可能作为甲状腺癌基因诊断、治疗的新靶点。
Objective To detect the expression of PTEN and Ki-67 in thyroid carcinoma and its clinical significance. Methods 40 cases of paraffin-embedded tissues and 14 cases of post-operation excised tissues were collected from the First People’s Hospital of Tianshui City, Gansu Province between September 2012 and March 2015 after pathological resection of the primary thyroid cancer. The expressions of PTEN, Ki-67 protein and m RNA in thyroid cancer tissues and corresponding paracancerous tissues were detected by immunohistochemical SP method and RT-PCR. The expressions of PTEN and Ki-67 protein and the clinicopathological parameters of thyroid cancer The relationship between. Results The positive rate of PTEN protein expression in thyroid cancer tissues was significantly lower than that in adjacent tissues (35.0% (14/40) vs 60.0% (24/40), P <0.05) 67 protein in thyroid cancer was significantly higher than that in the corresponding paracancerous tissues (72.5% (29/40) vs 42.5% (17/40), P <0.05). The relative gray value of PTEN m RNA expression in 14 cases of thyroid carcinoma was significantly lower than that in the corresponding paracancerous tissue (0.225 7 ± 0.036 3 vs 0.503 6 ± 0.037 5, P <0.05); Ki-67 m The relative gray value of RNA expression in 14 cases of thyroid cancer specimens was significantly higher than that in the corresponding paracancerous tissues (1.212 1 ± 0.042 1 vs 0.293 6 ± 0.027 4, P <0.05). The expression of PTEN and Ki-67 in thyroid cancer was correlated with histological grade, pathological type, tumor stage and regional lymph node metastasis (P <0.05), but not with sex, age and tumor envelope of thyroid cancer (P> 0.05). The expression of PTEN protein and Ki-67 protein in thyroid cancer was negatively correlated (rs = -0.605, P = 0.000), but not in the corresponding paracancerous tissue (rs = -0.021, P = 0.899). Conclusions The abnormal expression of PTEN and Ki-67 in thyroid cancer may be related to the occurrence, development and mechanism of thyroid cancer. The combination of PTEN and Ki-67 may contribute to the identification of pathological types of thyroid cancer and the judgment of biological behavior And clinical stage, may serve as a new target for the diagnosis and treatment of thyroid cancer gene.