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目的 探讨SARS恢复期患者特异性IgG抗体滴度水平及变化趋势 ,完善SARS感染后免疫的资料。方法 采用ELISA法检测 2 3例SARS患者发病后 6个月、1 2个月 2个时间点血清中特异性IgG抗体滴度。以单克隆抗体标记 2 3例患者的PBMC ,用流式细胞仪检测HLA A2的阳性率。结果 6个月时IgG抗体滴度的最高值为 1∶1 6 0阳性(4 / 2 3) ,最低值为 1∶1 0阴性 (1 / 2 3) ,平均滴度为 1∶5 7;1 2个月时IgG抗体的最高滴度仅为 1∶80阳性 (6 / 2 3) ,最低值为 1∶1 0阴性 (2 / 2 3) ,平均滴度为 1∶2 7。 2个时间点抗体滴度之间的差异有统计学意义(P =0 0 0 2 )。 2 3例患者中HLA A2阳性 1 4例 ,阳性率 6 0 9%。结论 SARS恢复期患者的抗体滴度在半年以后已呈现明显的下降趋势 ,目前所依据的SARS IgG抗体诊断标准是合理可行的。HLA A2抗原肽疫苗可以治疗和预防 5 0 %左右的SARS病毒感染。
Objective To investigate the level and trend of specific IgG antibody titers in SARS convalescent patients and to improve the data of immune response after SARS infection. Methods Serum specific IgG antibody titers were detected by ELISA at 6 months and 12 months after onset in 23 SARS patients. Twenty-three PBMCs were labeled with monoclonal antibody and the positive rate of HLA A2 was detected by flow cytometry. Results The highest IgG antibody titer at 6 months was 1: 1 60 positive (4/2 3), the lowest was 1: 1 0 negative (1/2 3) with an average titer of 1: 57. The highest titer of IgG antibody at 12 months was only 1:80 (6/2) and the lowest was 1: 1 0 negative (2/23) with an average titer of 1: 27. There was a significant difference in antibody titers between the two time points (P = 0.00002). Of the 23 patients, 14 were HLA A2 positive, with a positive rate of 60.9%. Conclusions The antibody titers of SARS convalescent patients have a clear downward trend after six months, and the diagnostic criteria of SARS IgG antibodies based on them are reasonable and feasible. HLA A2 antigen peptide vaccine can treat and prevent about 50% of SARS virus infection.