论文部分内容阅读
目的 观察甘氨脱氧胆酸 (GDC)对大鼠肝细胞钙激活性钾通道 (KCa)开放概率的影响。方法 体外培养大鼠肝细胞 ,以 GDC为处理因素 ,应用膜片钳技术通过贴附式和内面向外式膜片测定大鼠 KCa开放概率。结果 1细胞贴附式膜片下 ,浴液中 Ca2 +浓度 10 - 7mol/ L,GDC终浓度为 5 0 ,10 0 ,2 5 0 μmol/ L 时 ,概率由 0 .0 38± 0 .0 9分别增加到 0 .6 2 0±0 .338,0 .5 91± 0 .16 3,0 .895± 0 .176 (n=4,P均 <0 .0 1)。浴液中不加 GDC,改变 Ca2 +浓度 (10 - 7、10 - 6、10 - 5 mol/ L) ,KCa的开放概率并不发生明显改变 ,而在此液中加入 GDC(终浓度 10 0 μmol/ L) ,KCa开放概率由 0 .0 42± 0 .0 15增加到 0 .385±0 .330和 0 .5 5 1± 0 .16 3(n=4,P均 <0 .0 5 )。浴液中不含 Ca2 + ,并加入 EGTA,GDC浓度为 5 0 ,10 0 ,2 5 0 μmol/ L,KCa开放概率显著增加 (n=4,P均 <0 .0 5 ) ,但比浴液中有 Ca2 + 时增加程度小。 2内面向外式膜片下 ,浴液中无 Ca2 + ,加入 GDC(10 ,5 0 ,10 0 ,2 5 0μmol/ L ) ,KCa开放概率无明显改变 (n=2 ,P>0 .0 5 )。结论 1GDC对 KCa有明显的激活作用 ,并有浓度依赖性。 2 GDC既有引起胞外 Ca2 +内流 ,也能诱导内贮 Ca2 +的释放。 3GDC对 KCa的激活是通过 Ca2 +实现的。
Objective To observe the effect of glycodeoxycholic acid (GDC) on the open probability of calcium-activated potassium channel (KCa) in rat hepatocytes. Methods The rat hepatocytes were cultured in vitro. GDC was used as the treatment factor. The open probability of KCa in rats was measured by patch-clamp technique and in-plane outward patch. Results The results showed that under the patch attached membrane, the concentration of Ca2 + in the bath was 10-7mol / L, the final concentration of GDC was 50, 100 and 250μmol / L, the probability was increased from 0.038 ± 0.0 9 increased to 0.62 ± 0.338,0.591 ± 0.16 3,0 .895 ± 0.176 (n = 4, P <0.01), respectively. No GDC was added into the bath, and the Ca2 + concentration (10 - 7,10 - 6,10 - 5 mol / L) was changed without any significant change in the open probability of KCa. GDC (final concentration 10 0 μmol / L), the open probability of KCa increased from 0.042 ± 0.015 to 0.385 ± 0.330 and 0.55 1 ± 0.16 3 (n = 4, P <0.05) ). There was no Ca2 + in the bath and EGTA was added. The GDC concentrations were 50, 100 and 250 μmol / L, and the open probability of KCa was significantly increased (n = 4, P <0.05) There is little increase in Ca2 + in the liquid. 2 without Ca2 + in the bath and with GDC (10, 50, 100, 250 μmol / L), there was no significant change in the open probability of KCa (n = 2, P> 0 .0 5). Conclusions 1GDC can obviously activate KCa in a concentration-dependent manner. 2 GDC both cause extracellular Ca2 + influx, but also induce the release of Ca2 +. The activation of KCa by 3GDC is achieved by Ca2 +.