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目的 探讨阳离子转运蛋白2(0CT2)基因位点A270S(rs316019)不同基因型与成年人使用万古霉素造成急性肾损伤(AKI)的关系.方法 选择114例使用万古霉素的中国汉族患者,根据疗程中是否出现AKI,将其分为AKI组(35例)和无AKI组(79例).抽取患者外周静脉血,选用限制性片段长度多态性方法检测OCT2A270S基因多态性,用Logistic回归分析考察AKI的风险因素.结果 A270S基因型分布符合Hardy-Weinberg平衡(P＞0.05).AKI组A270SGG、GT和TT 基因型患者分别为26例(74.29％),8例(22.89％)和1例(2.82％),无AKI组A270S GG、GT和TT基因型患者分别为72例(91.94％),6例(7.59％)和1例(0.47％),差异均有统计学意义(均P＜0.05).Logistic回归结果显示,年龄、体重指数和A270S基因多态性均为万古霉素导致AKI的风险因素(均P＜0.05).结论 患者年龄、体重指数和A270S基因多态性可能与万古霉素导致的AKI有关.“,”Objective To analyze the association of vancomycin associated acute kidney injury (AKI) with gene polymorphisms of organic cation transporters 2 (OCT2) A270S.Methods A total of 114 Han inpatients using vancomycin were selected.According to the occurrence of AKI,those patients were divided into AKI group (35 cases)and non-AKI group(79 cases).Peripheral venous blood of patients were collected,polymerase chain reaction-restriction fragment length polymorphism were used to detect A270S gene polymorphisms.The susceptibility factors were investigated by Logistic regression analysis.Results A270S genotype distribution met Hardy-Weinberg balance (P ＞ 0.05).There were 26 patients (74.29％) with A270S (G/G) genotype,8 patients (22.89％)with A270S (G/T) genotype and 1 patients (2.82％) with A270S (T/T) genotype in AKI group.There were 72 patients (91.94％) with A270S (G/G) genotype,6 patients (7.59％) with A270S(G/T) genotype and 1 patients (0.47％) with A270S (T/T) genotype in non-AKI group.There was statistical significance in the distribution of A270S(G/G) and A270S (G/T),A270S(T/T) genotype between 2 groups (all P ＜ 0.05).Logistic regression analysis showed that there was correlation of age,body mass index (BMI) and A270S genotype with AKI (all P ＜ 0.05).Conclusion Age,BMI and A270S genotype may be correlated with vancomycin associated AKI.