曾报道柴朴汤具有抑制大鼠肥大细胞释放组胺的作用,且此作用主要归于方中成分日本厚朴提取物的作用。本次研究了日本厚朴提取物及其成分对化合物48/80(C48/80)诱导的大鼠腹膜肥大细胞释放组胺的影响。 材料和方法:①70g日本厚朴用700mL沸水回流提取2h后,过滤冻干,得到干燥提取物备用。5.0g干燥提取物上Diaion HP-20柱,分别用甲醇、50％甲醇洗脱, It has been reported that Chai Pu Tang has the effect of inhibiting the release of histamine from mast cells in rats, and this effect is mainly attributed to the effect of Fang Zhifang Magnolia officinalis extract. This study investigated the effects of extracts from Magnolia officinalis and its components on histamine release from rat peritoneal mast cells induced by compound 48/80 (C48/80). Materials and Methods: 170 g of Japanese Magnolia officinalis was extracted with refluxing 700 mL of boiling water for 2 h, filtered and lyophilized to obtain a dry extract. 5.0 g dry extract was applied to a Diaion HP-20 column and eluted with methanol and 50% methanol, respectively.