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目的分析辽宁省汉城型汉坦病毒(HV)的基因亚型及其分布情况。方法收集辽宁省内主要肾综合征出血热(HFRS)流行地区的鼠肺和病人血清标本,采用间接免疫荧光法(IFA)检测鼠肺中HV抗原,应用HV特异性引物以RT-PCR方法扩增标本中M和S基因片段的特异性核苷酸序列,测序后与已知病毒序列进行比较分析,构建系统发生树,以明确其型别和亚型及其地理分布情况。结果辽宁省HFRS疫区褐家鼠携带的病毒均为汉城型病毒(SEOV);对鼠肺及病人携带HV的部分M片段及部分S片段进行核苷酸序列分析表明,发现SEOV的同源性较高,变异较小,稳定性较高,而且地理位置相近地区基因组核苷酸序列的同源性很高(>98.8%),具有小范围的地理聚集现象。结论辽宁省流行的SEOV均为S3亚型,基因亚型分布比较单一。
Objective To analyze the genotype and distribution of Hantaan virus (HV) in Liaoning province. Methods Serum samples of murine lungs and patients were collected from the prevalence areas of major hemorrhagic fever with renal syndrome (HFRS) in Liaoning Province. HV antigen was detected by indirect immunofluorescence (IFA) in the lung of the patients with HFRS. The HV specific primers were amplified by RT-PCR The specific nucleotide sequences of the M and S gene fragments were amplified and compared with the known virus sequences after sequencing to construct a phylogenetic tree to clarify the types and subtypes and their geographical distribution. Results The viruses carried by Rattus norvegicus in HFRS epidemic area in Liaoning province were all serovar Typhi (SEOV). Nucleotide sequence analysis of some M fragments and some S fragments carried by HV in lungs and patients showed that the homology of SEOV was found Higher, less variation, higher stability, and high homology (> 98.8%) of the nucleotide sequences of the genomic regions with similar geographical locations with a small geographical aggregation. Conclusion The prevalence of SEOV in Liaoning Province is all of S3 subtypes.