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目的评估急性肝坏死兔甘氨酸结合对氨基苯甲酸（ＰＡＢＡ）转变成马尿酸的代谢能力。方法３０只雄性大耳白兔随机分组成实验组（２０只）用Ｄ－氨基半乳糖致大耳白兔急性肝坏死，对照组（１０只）注射生理盐水。采用高效液相色谱法测定血清中不同时间的对乙酰氨基马尿酸（ＰＡＡＨＡ）、对氨基马尿酸（ＰＡＨＡ）和对乙酰氨基苯甲酸（ＰＡＡＢＡ）的含量。结果实验组与对照组比较，马尿酸的形成明显减少。在不同时间分析马尿酸的代谢率，显示与肝损伤的程度相关，而ＰＡＢＡ的乙酰化程度与肝损伤程度无明显相关，在严重急性肝损伤时ＰＡＨＡ的浓度为零。结论甘氨酸结合ＰＡＢＡ的代谢率的测定可作为肝功能定量试验和急性肝坏死的灵敏指标。
Objective To assess the metabolic capacity of glycine-bound p-aminobenzoic acid (PABA) to hippuric acid in acute hepatic necrosis rabbits. Methods Thirty male large white rabbits were randomly divided into experimental group (n = 20) and acute hepatic necrosis (n = 6). The control group (n = 10) received normal saline. The content of PAHHA, PAHA and PAABA in serum at different time were determined by HPLC. Results Compared with the control group, the formation of hippuric acid in experimental group was significantly reduced. The metabolic rate of hippuric acid was analyzed at different times and showed a correlation with the degree of liver injury. However, the degree of acetylation of PABA was not significantly correlated with the degree of liver injury, and PAHA concentration was zero in severe acute liver injury. Conclusion The determination of the metabolic rate of glycine combined with PABA can be used as a sensitive indicator of quantitative liver function tests and acute liver necrosis.