人红细胞(RBC)在外周血循环中的平均寿命约120天。关于红细胞衰老问题,近年来争论较多的是老化RBC如何被吞噬细胞及网织内皮系统识别而被清除。最近有人提出老化RBC表面结合自身IgG,其Fc部分被吞噬细胞识别而被吞噬,但缺乏直接的证据。我们用纯化的人IgG分别与年轻及老化RBC膜(用Ficoll密度梯度高心获得年轻及衰老RBC)于37℃保温30min,然后与辣根过氧化物酶标记 The average life span of human erythrocytes (RBCs) in the peripheral circulation is about 120 days. On the issue of red cell aging, in recent years more controversial is how aging RBC was phagocytic cells and reticuloendothelial system to identify and be cleared. Recently, it was suggested that the surface of aging RBC bound to its own IgG, the Fc part of which was phagocytized cells to be phagocytosed, but the lack of direct evidence. Purified human IgG was incubated with young and aged RBC membranes (young and aged RBCs with a high Ficoll density gradient) at 37 ° C for 30 min and then incubated with horseradish peroxidase