在高压电脉冲作用下,细胞膜脂双分子层形成可通透的暂时性微孔,这种过程和方法称为电击或电穿孔。在植物细胞方面,电击基因转移的应用大多以原生质体为受体。由于原生质体的酶解过程使细胞受损和很多植物原生质体植株再生仍困难,因此限制了这一技术的应用。所以,以完整的不去壁的植物细胞作为受体,进行电击基因转移便有重要意义。很多基因转入受体细胞后不表达或在以后的世代中丢失是限制基因工程技术发展的又一重要原 Under the action of high-voltage electric pulse, the cell membrane lipid bilayer forms a permeable and temporary micropore, a process and method called electric shock or electroporation. In plant cells, most of the applications of electric shock gene transfer take protoplasts as receptors. Because of the protoplast enzymatic process of cell damage and many plant protoplast regeneration is still difficult, thus limiting the application of this technology. Therefore, the complete non-wall plant cells as a receptor for shock gene transfer is of great significance. It is another important factor that limits the development of genetic engineering technology when many genes are not expressed in recipient cells or lost in later generations