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本文报道了采用~3H—胸腺嘧啶核苷(~3H—TdR)掺入法及酸性α—醋酸萘酯酶(ANAE)染色法分别检测了各组大白鼠的淋巴细胞转化率和T淋巴细胞计数,用聚乙二醇(PEG)沉淀法检测了各组的循环免疫复合物(CIC),同时测定了各组的肝硒含量,结果表明,病区粮组的淋转率明显低于非病区粮组(P<0.02);病区粮加硒组和病区组相比有所提高:但不明显(P>0.2);病区粮组和加硒粮组的T淋巴细胞计数均低于非病区粮组(P<0.005)。病区粮组CIC及肝硒含量均低于其他两组(P<0.02),提示:病区粮因素可导致大白鼠的细胞免疫功能降低,病区粮加硒并不能完全纠正病区粮中的影响因素。
In this paper, the lymphocyte transformation rate and T lymphocyte count of rats in each group were detected by ~ 3H-thymidine incorporation and ANAE staining. . The circulating immunocomplexes (CICs) of each group were detected by polyethylene glycol (PEG) precipitation method. The hepatic and selenium contents in each group were also determined. The results showed that the rate of lymphatic metastasis in the ward group was significantly lower than that in the non- (P <0.02). Compared with the diseased area group, the dietary supplementation with selenium in the diseased area increased but was not obvious (P> 0.2). The T lymphocyte counts of the diseased area and the selenium-enriched grain group were all lower In non-ward food group (P <0.005). The CIC and hepatic and selenium contents in the ward food group were lower than those in the other two groups (P <0.02), suggesting that the ward grain factors may lead to the decrease of cellular immune function in the ward rats. The impact of factors.