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小麦单细胞培养研究于1985~1995年进行,以六倍体普通小麦84—53的幼穗为外植体.在诱导胚性愈伤组织基础上.经振荡悬浮培养获得单细胞再生植株,建立体细胞无性系,并系统研究再生植株后代体细胞无性系的变异规律和遗传稳定性、体细胞无性系有利变异的选择及其在小麦品种改良中应用的可能性.本文报道有关单细胞培养和再生植株的研究结果.
Single cell culture of wheat was conducted from 1985 to 1995. The young spikes of hexaploid wheat 84-53 were used as explants. On the basis of inducing embryogenic callus. The single cell regenerated plants were obtained by oscillatory suspension culture, and the somatic cell clones were established. The variation regularity and genetic stability of somatic cell clones and the favorable mutation of somatic cell clones were systematically studied and their application in the wheat variety improvement The possibility of. This paper reports the results of studies on single cell cultures and regenerated plants.