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[目的]对47份菌草种质资源进行遗传多样性分析。[方法]利用iPBS(Inter Primer Binding Site Amplification,引物结合位点间扩增)分子标记技术对47份菌草种质资源材料进行遗传多样性分析。利用从28个iPBS引物中共挑选出的11个扩增效果较好的iPBS引物,对47份菌草种质材料的DNA进行PCR扩增和电泳分析。[结果]11个iPBS引物在47份菌草种质材料上共给出了208个多态片段,平均每个引物给扩增出18.9个多态片段,聚类分析表明,供试材料之间的遗传相似性系数(SM)分布在0.58~0.99之间,在遗传相似系数0.67时可将47份菌草种质材料分成10大类群,所有47份材料被区分开来。[结论]iPBS分子标记技术可以有效用于菌草种质资源材料的品种鉴定和遗传多样性分析,本研究为科学管理和利用菌草种质资源提供理论指导和技术支撑。
[Objective] The genetic diversity of 47 germplasm resources of Myrica gratiaria was analyzed. [Method] The genetic diversity of 47 grass germplasm resources was analyzed by iPBS (Inter Primer Binding Site Amplification) molecular marker technique. A total of 11 iPBS primers amplified from 28 iPBS primers were used to amplify and analyze the DNA of 47 germ plasm materials. [Result] A total of 208 polymorphic fragments were obtained from 11 iPBS primers on 47 species of germplasm resources, with an average of 18.9 polymorphic fragments amplified by each primer. Cluster analysis showed that between the tested materials The genetic similarity coefficient (SM) was between 0.58 and 0.99. At genetic similarity coefficient of 0.67, 47 species of Myrica gracilica germplasm could be divided into 10 groups and all 47 species were distinguished. [Conclusion] The iPBS molecular marker technology could be used effectively to identify the species and genetic diversity of Mycorrhizal fungi germplasm resources. This study provided theoretical guidance and technical support for the scientific management and utilization of mycological germplasm resources.