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[目的]考察三代小鼠十溴联苯醚(decabromodiphenyl ether,BDE 209)灌胃染毒对两代子鼠空间学习记忆能力和空间位置记忆能力的影响。[方法]将75只雌性昆明F0代小鼠随机分为对照组[0 mg/(kg·d)]、1.5 mg/(kg·d)和225 mg/(kg·d)染毒组,每组25只,BDE 209灌胃10 d后,与雄鼠合笼,每组选取怀孕时间相近(相差不超过2 d)的8只母鼠孕期持续染毒至子鼠断乳。第一代(F1)及第二代(F2)子鼠在断乳后也按照与母鼠相同的方式进行染毒。观察出生后子鼠数量并测量第30、60和90天子鼠体重,并利用Morris水迷宫评价出生后第30、60和90天子鼠的空间学习记忆能力和空间位置记忆能力。[结果]与对照组比较,1.5 mg/(kg·d)组F0和F1代母鼠产仔数量分别降低约25%、30%(P<0.05);225 mg/(kg·d)组F1代出生后30天雌性子鼠体重降低约17%(P<0.05);染毒组F1代出生后30天雄性子鼠体重分别降低约12%(P<0.05)、25%(P<0.01);染毒组F2代出生30天雌性子鼠体重降低约12%(P<0.01);1.5 mg/(kg·d)组F2代出生后30天雄性子鼠体重降低约8%(P<0.05),225 mg/(kg·d)组F2代出生后60、90天雄性子鼠体重均降低约13%(P<0.01);各组F1代60、90天,F2代30、60和90天雌性子鼠逃避潜伏期延长,结果具有统计学意义(P<0.05或P<0.01);F1代1.5 mg/(kg·d)组出生后60天,各组出生后90天,及F2代225 mg/(kg·d)组出生后30、60、90天雄性子鼠逃避潜伏期延长,结果具有统计学意义(P<0.05或P<0.01);F1、F2代子鼠平台象限活动时间差异无统计学意义(P>0.05);F2代出生后60和90天雌性子鼠平台穿越次数减少,结果具有统计学意义(P<0.05或P<0.01);F2代雄性子鼠出生后90天平台穿越次数减少,结果具有统计学意义(P<0.05)。[结论]三代小鼠BDE 209染毒能够影响子代发育,降低子代空间学习记忆能力和空间位置记忆能力。
[Objective] To investigate the effects of three generations of decabromodiphenyl ether (BDE 209) on spatial learning and memory ability and spatial location memory ability in mice. [Methods] Seventy-five female Kunming F0 mice were randomly divided into control group [0 mg / (kg · d)], 1.5 mg / (kg · d) and 225 mg / (kg · d) (N = 25). BDE 209 was intragastrically administered for 10 days and then caged with male rats. Eight females with similar gestational age (no more than 2 days apart) were continuously exposed to weanling during pregnancy. The first generation (F1) and second generation (F2) offspring were also exposed to the same manner as the female after weaning. The number of offspring after birth was observed and the weights of rats on the 30th, 60th and 90th days were measured. Morris water maze was used to evaluate the spatial learning and memory ability and spatial memory ability of the 30th, 60th and 90th days after birth. [Results] Compared with the control group, the litter size of F0 and F1 offspring of 1.5 mg / (kg · d) group decreased by about 25% and 30%, respectively (P <0.05) The body weight of female offspring was reduced by about 17% (P <0.05) 30 d after birth. The weight of male offspring of F1 generation decreased about 12% (P <0.05) and 25% (P <0.01) (P <0.01). The body weight of the male offspring decreased by about 12% (P <0.05) in the F2 generation on the F2 generation after exposure to F2 for 30 days in the F2 generation, ), 225 mg / (kg · d) of F2 generation on the 60th and 90th day after birth, the body weight of male mice decreased by about 13% (P <0.01) (P <0.05 or P <0.01). In the F1 generation, the 1.5 mg / (kg · d) group was 60 days after birth, the groups were born 90 days after birth and the F2 generation 225 The escape latency of male mice at 30, 60 and 90 days after birth was significantly longer in mg / (kg · d) group, with statistical significance (P <0.05 or P <0.01). (P <0.05 or P <0.05). The passage number of female offspring decreased on the 60th and 90th days after birth in F2 generation, with statistical significance (P <0.05 or P <0.01). After F2 generation male mice were born The number of crossings of 90-day platform decreased, the result was statistically significant (P <0.05). [Conclusion] The BDE 209 strain of three generations of mice could affect the development of offspring, reduce the ability of spatial learning and memory and spatial location memory of offspring.