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目的:探讨清毒颗粒对二甲基苯蒽(DMBA)诱导SD大鼠乳腺癌的药效。方法:DMBA诱导大鼠乳腺癌,观察清毒颗粒对肿瘤抑制率,HE染色观察病理特点,IHC检测肿瘤组织Ki-67分布及含量,ELISA检测大鼠血清中IL~-12、IFN-γ、IL-4和IL~-10含量。结果:低、中、高剂量清毒颗粒和柴胡皂苷a的抑瘤率分别为:30.93%,43.84%,44.17%和43.48%;清毒颗粒和柴胡皂苷a可显著降低Ki-67表达量;上调血清中IL~-12、IFN-γ含量,下调IL-4、IL~-10含量。结论:清毒颗粒具有抗乳腺癌作用,其机制可能是调节机体免疫,增强抗肿瘤免疫作用。
Objective: To investigate the effect of Qingdu Granules on DMBA-induced breast cancer in SD rats. Methods: The rat breast cancer was induced by DMBA. The tumor inhibition rate was observed by Qingdu Granule. The pathological characteristics were observed by HE staining. The distribution and content of Ki-67 in tumor tissue were detected by IHC. The levels of IL-12 and IFN- IL-4 and IL ~ -10 content. Results: The inhibitory rates of low, middle and high doses of Qingdu Granules and Saikosaponin a were 30.93%, 43.84%, 44.17% and 43.48%, respectively. Qing-kyu granule and saikosaponin a could significantly reduce the expression of Ki-67 The levels of IL-12 and IFN-γ in serum were increased, and the contents of IL-4 and IL-10 were also decreased. Conclusion: Qingdu granule has anti-breast cancer effect, and its mechanism may be to regulate body immunity and enhance antitumor immunity.