论文部分内容阅读
以樱桃组培苗‘吉塞拉5号’为试材,采用营养液水培控制溶氧浓度的方法,研究短期低氧胁迫下钙对其根系功能及氮代谢的影响。结果表明:与对照相比,低氧加钙处理的樱桃植株根系活力显著升高,根系呼吸速率抑制作用明显减轻,与氮代谢相关的硝酸还原酶(NR)、谷氨酰胺合成酶(GS)及谷氨酸脱氢酶(NADH-GDH)活性也显著升高,从而为低氧逆境下樱桃根系的吸收作用提供足够的能量,保证了糖酵解和电子传递的顺利进行,缓解了樱桃的低氧伤害,且10mmol·L-1Ca2+处理的缓解效果优于5mmol·L-1处理;低氧缺钙处理的樱桃植株根系活力显著下降,根系呼吸速率抑制作用明显加重,NR、GS及NADH-GDH活性显著降低;表明适当提高生长介质中的Ca2+浓度可缓解低氧胁迫对樱桃根系的伤害。
Using cherry hydroponic culture ’Gisela 5’ as test material, hydroponic culture was used to control the concentration of dissolved oxygen, and the effects of calcium on its root function and nitrogen metabolism under short-term hypoxia stress were studied. The results showed that compared with the control, the root activity of the roots treated with hypoxia and calcium increased significantly, and the inhibition of root respiration rate was significantly reduced. Nitrate reductase (NR) and glutamine synthetase (GS) And glutamate dehydrogenase (NADH-GDH) activity was also significantly increased, so as to hypoxia stress the absorption of the roots of cherry to provide sufficient energy to ensure the smooth progress of glycolysis and electron transfer, relieve the cherry Hypoxia injury, and 10mmol·L-1Ca2 + treatment better than 5mmol·L-1 treatment; hypoxia-deficient calcium treatment of root activity decreased significantly, root respiration rate inhibition was significantly increased, NR, GS and NADH- GDH activity was significantly reduced; indicating that proper increase of Ca2 + in growth medium can alleviate the hypoxia stress on cherry root injury.