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为了快速检出ＡＰＣ基因突变，应用选择性检测产生终止密码突变的体外蛋白合成试验（ＩＶＳＰ），对１１５例Ｎｏｎ－ＦＡＰ大肠肿瘤标本进行了研究。发现７６例１０７条不全蛋白条带，序列分析证实９４％（１０１／１０７）为突变带，其中６条非特异性带可能与片段内ＡＴＧ启动子存在有关。为排除小分子量ＡＰＣ不全蛋白的遗漏，对各片段起始部的４００直接行序列分析均未检出突变点。结果显示，ＩＶＳＰ不仅是迄今最为快速、敏感和特异的ＡＰＣ突变检测方法之一，而且还有助于突变的定性（病理性）和定位。
In order to rapidly detect APC gene mutations, an in vitro Protein Synthesis Assay (IVSP) was performed to selectively detect non-FAP colorectal tumors. Seventy-seven samples of 107 incomplete protein bands were found. Sequence analysis confirmed that 94% (101/107) of the bands were mutational bands, of which 6 non-specific bands may be related to the presence of the ATG promoter in the fragments. To exclude the omission of small-molecular-weight APC incomplete proteins, no mutations were detected in the 400 direct sequence analysis of the start of each fragment. The results show that IVSP is not only one of the fastest, sensitive and specific APC mutation detection methods to date, but also contributes to the qualitative (pathological) and localization of mutations.