论文部分内容阅读
目的探讨人心房利钠肽(h ANP)对小鼠机械通气所致肺损伤(VILI)时炎症反应的影响。方法将30只雄性C57 B/6小鼠随机分为空白对照组(C组)、机械通气肺损伤组(MV组)和人心房利钠肽组(MV+h ANP组)。MV+h ANP组腹腔注射h ANP 2μg/kg后行机械通气,通气3 h后取材,处死小鼠。采用流式多重蛋白定量技术(CBA)测定小鼠血浆中炎性细胞因子浓度;分离左肺,计算肺组织湿干比值。结果与C组相比,MV组、HV+h ANP组血浆中巨噬细胞炎性蛋白-1[(461.7±91.97)、(348.0±74.40)pg/ml]、肿瘤坏死因子-α[(59.10±7.94)、(48.40±8.34)pg/ml]和白细胞介素-6[(701.7±88.13)、(607.9±61.67)pg/ml]含量明显上升(P<0.05),肺组织W/D值[(4.74±0.84)、(4.61±0.721)]明显升高(P<0.05);与MV组相比,HV+h ANP组血浆中巨噬细胞炎性蛋白-1、肿瘤坏死因子-α、白细胞介素-6明显降低(P<0.05),肺组织W/D降低(P<0.05)。结论人心房钠尿肽能够减轻机械通气所导致的炎症反应,从而减轻肺损伤。
Objective To investigate the effects of human atrial natriuretic peptide (h ANP) on the inflammatory response induced by mechanical ventilation-induced lung injury (VILI) in mice. Methods Thirty male C57 B / 6 mice were randomly divided into blank control group (C group), MV group (MV group) and human atrial natriuretic peptide group (MV + h ANP group). MV + h ANP group were intraperitoneally injected with h ANP 2μg / kg after mechanical ventilation, ventilation 3h after the material taken, the mice were sacrificed. The concentration of inflammatory cytokines in the plasma of mice was determined by flow cytometry (CBA). The left lung was separated and the ratio of wet to dry lung tissue was calculated. Results Compared with group C, the levels of macrophage inflammatory protein-1 [(461.7 ± 91.97), (348.0 ± 74.40) pg / ml] and tumor necrosis factor-α (P <0.05). The contents of W / D in lung tissue were significantly higher than those in control group (P <0.01) (4.74 ± 0.84), (4.61 ± 0.721)] in HV + h ANP group (P <0.05). Compared with MV group, the plasma levels of macrophage inflammatory protein-1, tumor necrosis factor- Interleukin-6 was significantly decreased (P <0.05), and W / D in lung tissue was decreased (P <0.05). Conclusion Human atrial natriuretic peptide can reduce the inflammatory reaction induced by mechanical ventilation and reduce lung injury.