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本文通过对稻曲病菌在不同培养基、不同营养条件（碳源和氮源）、不同ｐＨ值、不同温度和光照及不同代数的菌株在ＰＳＡ上产孢能力的研究，初步明确，ＰＳＡ是适宜的产孢培养基；２～３代菌株最易产孢；ＮＨ４ＣＩ和ＫＮＯ３对厚垣孢子产生有一定的作用；适宜产孢的温度为１５～２５℃，但１５℃和２０℃培养时间较长；培养基适宜的ｐＨ值是５～７，以ｐＨ６为最佳；光照对厚垣孢子的产生数量及时间都有很大的影响，光暗交替是适宜的培养条件。
In this paper, through the research on spore forming ability of Ustilauginulae vaginalis strains on PSA in different media, different nutrient conditions (carbon and nitrogen sources), different pH values, different temperature and light and different algebra strains, it is initially clear that PSA is suitable Of sporulation medium. The 2nd and 3rd generation strains were the most susceptible to sporulation. NH4CI and KNO3 had a certain effect on the chlamydospore production. The suitable sporulation temperature was 15-25 ℃, but the culture time was longer at 15 ℃ and 20 ℃ The suitable pH value of culture medium was 5 ~ 7, and pH 6 was the best. The quantity and time of chlamydospore production were greatly affected by light. The light and dark alternation was the suitable culture condition.