论文部分内容阅读
目的:观察益气活血中药对大鼠右肺辐照后外周血T淋巴细胞亚群的影响。方法:54只Wistar种雌性大鼠随机分为正常对照组(A组)6只、单纯照射组(B组)24只、照射加中药组(C组)24只。6MV-X直线加速器对B、C组大鼠右肺进行分次照射(5Gy/次,1次/周,总剂量30Gy),于照射后第6、8、12、26周处死,每组各6只大鼠,A组6只于第6周末处死,取抗凝全血进行流式细胞检测CD4+、CD+8、CD4+/CD+8的动态变化。结果:B组大鼠各时相点外周血CD4+/CD+8比值下降,显著性低于A组(P<0.05)。C组大鼠各时相点外周血中CD4+/CD+8比值均高于B组(P<0.05)。结论:益气活血中药具有减轻大鼠右肺辐照后外周血T淋巴细胞亚群的损伤作用且有助于恢复。
Objective: To observe the effect of Yiqi Huoxue Chinese medicine on T lymphocyte subsets of peripheral blood after irradiation of right lung in rats. Methods: Fifty-four Wistar female rats were randomly divided into normal control group (A group), 6 rats in group B (irradiation group B) and 24 rats in control group (C group). 6MV-X linear accelerator on the right lung of rats in groups B and C were divided into groups (5Gy / time, 1 time / week, total dose of 30Gy), and were sacrificed at 6,8,12,26 weeks after irradiation Six rats in group A were sacrificed at the end of the sixth week, and the whole blood was collected for anticoagulation for the dynamic changes of CD4 +, CD + 8 and CD4 + / CD + 8 by flow cytometry. Results: The ratio of CD4 + / CD + 8 in peripheral blood in B group decreased significantly at each time point, which was significantly lower than that in A group (P <0.05). The ratio of CD4 + / CD + 8 in peripheral blood in C group at each time point was higher than that in B group (P <0.05). Conclusion: Yiqi Huoxue Chinese medicine can attenuate the damage of T lymphocyte subsets in the peripheral blood after irradiation of the right lung in rats and help to recover.