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血管生成抑制素 (angiostatin)能特异地抑制新生血管生成 ,有潜在的临床应用价值 .利用亲和层析从人血浆中纯化纤溶酶原 ,并在原位进行弹性蛋白酶有限消化产生angiostatin片段 ,经过洗涤 ,然后用 0 2mol/L 6 氨基己酸溶液将angiostatin特异性洗脱 .此改进使整个制备过程变得简单、快速和高效 .体外对牛主动脉内皮细胞的生长抑制实验以及体内鸡胚尿囊膜血管生成抑制分析结果证实所制备的angiostatin有较强的抑制血管生成活性
Angiostatin can specifically inhibit neovascularization and has potential clinical application value.Prinolytic plasminogen is purified from human plasma by affinity chromatography, and angiostatin fragment is produced by in situ digestion with elastase. After washing, the angiostatin was eluted specifically with 0 2mol / L 6-aminocaproic acid solution.This improvement made the whole preparation process simple, rapid and efficient.The growth inhibition of bovine aortic endothelial cells in vitro and in vivo chick embryo The angiogenesis inhibition assay results of the allantoic membrane confirmed that the prepared angiostatin has a stronger anti-angiogenic activity