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从正常人不同发育时期 ,不明原因流产 ,增殖型和侵蚀型葡萄胎滋养细胞角度 ,用免疫组织化学方法观察纤维粘连蛋白 ( FN)的显微定位 ,比较研究其不同定位与滋养上皮增殖 ,生长 ,分化 ,凋亡 ,迁移和浸润的关系。结果显示 :正常人不同发育时期 ,F N在早孕合体滋养细胞基底膜和绒毛外滋养细胞膜呈阳性着色 ,在中期非合体结处的滋养细胞质呈免疫反应阳性 ,在足月滋养细胞呈阴性着色 ;不明原因流产 ,FN在合体滋养细胞核内呈阳性着色 ;FN在增殖型葡萄胎滋养细胞膜和绒毛外滋养细胞质呈强阳性着色 ;FN在侵蚀型葡萄胎滋养细胞质呈阳性着色。提示 FN胞质定位与滋养上皮迁移和侵蚀密切相关 ,FN基底膜定位与滋养细胞分化密切相关 ,FN胞膜定位与滋养细胞增殖相关 ,FN的阴性着色与滋养细胞衰老 ,FN胞核转位与滋养细胞凋亡可能相关
Fibrinolysis (FN) microscopic localization was observed by immunohistochemistry at different developmental stages of human normal, unexplained abortion, proliferative and hydatidiform mole trophoblast cells, compared with different location and nourishment epithelial proliferation and growth , Differentiation, apoptosis, migration and infiltration of the relationship. The results showed that at different developmental stages of normal people, FN positive staining of trophoblast cells in early pregnancy and trophoblastic villi and positive immunocytochemical staining of trophoblastic cytoplasm at mid-term non-junctional junction, and negative staining of full-term trophoblastic cells. FN in the syncytiotrophoblast nuclei positive staining; FN in fertilized hydatidiform mole trophoblastic membrane and villous trophoblast cytoplasm was strongly positive staining; FN in the hydatidiform mole trophoblast cytoplasm positive staining. These results suggest that the localization of FN is closely related to the migration and erosion of epithelial cells. The localization of FN basement membrane is closely related to the differentiation of trophoblast cells. The localization of FN is related to the proliferation of trophoblast cells. The negative staining of FN and the senescence of trophoblast cells, Trophoblastic apoptosis may be related