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鉴于目前国内各省、市的制剂手册上收载的链霉素滴眼液均无含量测定方法,同时链霉素的含量(效价)测定方法主要采用抗生素微生物检定法。此方法一般医院制剂室难以开展。本文即在文献的基础上,利用链霉素在碱性溶液中,经分子重排、消除反应后生成麦芽酚,可与铁离子(Fe~(+++))结合形成紫红色络合物的性质,采用比色法测定链霉素滴眼液的含量。方法简便、结果满意。一、仪器与试药 721型分光光度计(上海第三分析仪器厂) 链霉素国家标准品,北京药品生物制品检定所提供,批号:800811,1毫克相当于
In view of the current domestic provinces and municipalities manuals contained in the preparation of streptomycin eye drops have no content determination method, and the determination of streptomycin content (potency) method mainly uses antibiotics microbial assay. This method is difficult to carry out the general hospital preparation room. This article is based on the literature, the use of streptomycin in alkaline solution, the molecular rearrangement, eliminating the reaction of maltol, with iron ions (Fe ~ (+++)) to form a purple complex The nature of the colorimetric method for the determination of streptomycin eye drops content. The method is simple and the result is satisfactory. First, the instrument and reagent 721 spectrophotometer (Shanghai Third Analytical Instrument Factory) streptomycin national standard, provided by the Beijing Institute of Biological Products, batch number: 800811,1 mg equivalent to