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从活化的正常人外周血单核细胞中提取总ＲＮＡ，经逆转录多聚酶链反应（ＲＴＰＣＲ）获得了人白介素１受体拮抗剂（ＩＬ１Ｒａ）ｃＤＮＡ。经过ＤＮＡ测序分析，发现该片段和国外发表的ＩＬ１ＲａｃＤＮＡ序列一致。将该目的基因插入ｐＢＶ２２０载体，并转入大肠杆菌ＨＢ１０１中，经热诱导表达重组蛋白。ＳＤＳＰＡＧＥ后发现，菌体超声裂解后，在可溶性上清中有一Ｍｒ１７０００的特异带，约占菌体可溶性总蛋白的８０％以上。反复冻融裂菌后，上清经离子交换纯化获得电泳纯蛋白样品。ＥＬ４和ＣＴＬＬ细胞培养测定表明，得到的重组蛋白具有天然人ＩＬ１Ｒａ的生物活性。
Total RNA was extracted from activated normal human peripheral blood mononuclear cells and human interleukin 1 receptor antagonist (IL-1Ra) cDNA was obtained by reverse transcriptase-polymerase chain reaction (RT-PCR). After DNA sequencing analysis, we found that the fragment published abroad and IL 1RacDNA sequence. The gene of interest was inserted into pBV220 vector and transformed into E. coli HB101 to express the recombinant protein by heat induction. After SDS-PAGE, it was found that there was a Mr 17000 specific band in the soluble supernatant after the bacterial cells were lysed by ultrasound, accounting for more than 80% of the total bacterial soluble total protein. After repeated freeze-thaw cycles, the supernatant was purified by ion exchange to obtain electrophoretic pure protein samples. EL 4 and CTLL cell culture assays showed that the resulting recombinant protein has the biological activity of human IL 1Ra.