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采用HP20大孔吸附树脂、ODS、硅胶柱色谱、Sephadex LH-20柱色谱和半制备HPLC等色谱分离手段对紫花前胡的化学成分进行研究。结合理化性质及MS,NMR等谱学数据鉴定化合物的结构,从紫花前胡80%乙醇提取物中分离并鉴定了12个化合物,分别是(9R,10R)-9-acetoxy-8,8-dimethyl-9,10-dihydro-2H,8H-benzo[1,2-b:3,4-b’]dipyran-2-one-10-yl ester(1)、补骨脂呋喃香豆精(2)、顺式-3’,4’-二千里光酰基-3’,4’-二氢邪蒿内酯(3)、(3’R,4’R)-3’-angeloyloxy-4’-senecioyloxy-3’,4’-dihydroseselicalipteryxin(4)、(+)-8,9-dihydro-8-(2-hydro-xypropan-2-yl)-2-oxo-2H-furo[2,3h]chromen-9-yl-3-methylbut-2-enoate(5)、libanoridin(6)、丝立尼亭(7)、花椒素(8)、crocatone(9)、peujaponisinol B(10)、peujaponisinol A(11)、ostenol(12)。其中,化合物1~5为首次从当归属植物中分离得到,化合物7~12为首次从紫花前胡中分离得到。
The chemical constituents of P. purpurea were studied by HP20 macroporous adsorption resin, ODS, silica gel column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC. According to the physicochemical properties and MS, NMR and other spectroscopic data to identify the structure of compounds, 12 compounds were isolated and identified from 80% ethanol extract of Viola purpurea, namely (9R, 10R) -9-acetoxy-8,8- dimethyl-9,10-dihydro-2H, 8H-benzo [1,2-b: 3,4-b ’] dipyran-2-one- (3’R, 4’R) -3’-angeloyloxy-4 ’, cis-3’, 4’-dicenoyl- -senecioyloxy-3 ’, 4’-dihydroseselicalipteryxin (4), (+) - 8,9- dihydro- 8- (2-hydro- xypropan-2-yl) chromen-9-yl-3-methylbut-2-enoate (5), libanoridin (6), serotinin (7), capsaicin (8), crocatone (9), peujaponisinol B (10), peujaponisinol A 11), ostenol (12). Among them, compounds 1 to 5 were isolated from Angelica for the first time, and compounds 7 to 12 were isolated for the first time from P. purpurea.