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目的:研究氟保护漆应用于乳牙釉质表面对酸性含乳饮料致离体乳牙釉质脱矿的抑制作用,阐明氟保护漆的抗脱矿作用,为氟保护漆的临床推广及婴幼儿群防提供实验室依据。方法:将预备好的30个无龋、无明显裂纹及釉质发育缺陷的乳牙唇侧釉质块随机分为人工唾液A组(对照组)、B组(酸性含乳饮料组)、C组(0.1%氟保护漆组),每组10个标本。C组标本之釉质表面用小毛刷均匀涂布氟保护漆2次,自然晾干45 min。A组置于37℃恒温的10 mL人工唾液中浸泡4 d。B、C 2组在37℃恒温水箱中浸泡于10 mL酸性含乳饮料中,浸泡2 min,取出1 min,再浸泡,共9 min。3次/d,共4 d。3组牙釉质制作标本,应用场发射扫描电镜和能谱分析仪,检测乳牙釉质的表面形态变化,Ca2+、P3+含量数值(重量百分比)及Ca2+、P3+比值,采用SPSS 11.5软件包对数据进行统计学分析。结果:酸性含乳饮料浸泡可致乳牙釉质Ca2+、P3+含量显著降低(P<0.01)。氟保护漆处理后的釉质Ca2+、P3+含量降低程度显著低于酸性含乳饮料浸泡组(P<0.01)。结论:酸性含乳饮料对乳牙釉质表面有较强的酸蚀脱矿作用。釉质表面应用氟保护漆可增加硬度,在一定程度上抑制乳牙釉质在酸性含乳饮料中的脱矿,但不能完全抵御饮料的酸蚀。
OBJECTIVE: To study the inhibitory effect of fluoride protective paint on the enamel surface of deciduous enamel, and to study the demineralization of enamel demineralized from acidic milk-containing beverage in vitro, to elucidate the anti-demineralization effect of fluoride protective paint and to provide the clinical promotion of fluoride protective paint and the prevention of infants and young children Laboratory basis. Methods: Thirty labial deciduous enamel lumps without caries, no obvious crack and enamel development were randomly divided into three groups: the control group, the group B, the group containing acidic saliva, the group C, % Fluoride protective paint group), each group of 10 specimens. C group specimens enamel surface with a small brush evenly coated fluoride protective paint 2 times, dry naturally 45 min. Group A was immersed in 10 mL artificial saliva at a constant temperature of 37 ° C for 4 days. B and C 2 groups were soaked in 10 mL acidic milk-containing beverage at 37 ℃ in water tank for 2 min, removed for 1 min, then soaked for 9 min. 3 times / d, a total of 4 d. Three groups of enamel samples were made. The morphological changes, Ca2 +, P3 + content (% by weight) and Ca2 +, P3 + ratio of enamel of primary teeth were detected by field emission scanning electron microscope and energy spectrum analyzer. SPSS11.5 software package was used to analyze the data Analysis. Results: The soaking of acidic milk beverage could significantly decrease the contents of Ca2 + and P3 + in enamel of enamel (P <0.01). The content of Ca2 + and P3 + in enamel treated with fluorinated paint was significantly lower than that in acid-containing dairy beverage soaked group (P <0.01). Conclusion: Acidic milk drinks have a strong acid-etching ore demineralization on the enamel surface of deciduous teeth. Fluorine-lacquer enamel surface can increase the hardness, to a certain extent, inhibit the enamel demineralization in acid milk beverage demineralization, but can not completely resist the acid corrosion of beverages.