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目的 探究体内镉染毒对昆明小鼠骨髓造血干细胞线粒体功能的影响.方法 选用体质量(20±2)g雄性昆明小鼠,随机分为对照组、镉低剂量组(7.5 mg/kg)和镉高剂量组(15 mg/kg),镉低剂量组和镉高剂量组小鼠分别于每周一、三、五灌胃给予氯化镉溶液,对照组小鼠灌胃同等体积的蒸馏水,连续6周,于第8周提取小鼠骨髓细胞,利用流式细胞仪测定骨髓造血干细胞线粒体膜电位和线粒体活性氧(ROS)水平.结果 与对照组相比,镉低剂量、高剂量组小鼠体质量增长受到明显抑制(P<0.01);其造血干细胞线粒体ROS水平显著升高(P<0.05,P<0.01),且镉高剂量组线粒体ROS水平显著高于镉低剂量组(P<0.05);镉低剂量、镉高剂量组小鼠造血干细胞线粒体膜电位显著降低(P<0.05,P<0.01),且镉高剂量组线粒体膜电位显著低于镉低剂量组(P<0.05).结论 体内镉染毒引起昆明小鼠骨髓造血干细胞氧化损伤,导致线粒体功能异常,且与剂量相关.“,”Objective To explore the effect of cadmium chloride on mitochondrial function of hematopoietic stem cells in mouse bone marrow .Methods After being quarantined for one week , male Kunming mice weighted 20 ±2 g were randomly divided into three groups: control group , low dose cadmium-exposure group and high dose cadmium-exposure group.Mice in low dose and high dose cadmium-exposure groups were exposed to cadmium chloride solution at a dose of 7.5, 15 mg/kg body mass while those in control group were given an equal volume of distilled water through gavage administration every Monday , Wednesday and Friday for six consecutive weeks before cells in mouse bone marrow were collected at the 8th week.Mitochondrial membrane potential and ROS levels of mouse hematopoietic stem cells were detected using a flow cytometry .Results Compared with control group , the gain of body weight was significantly suppressed in cadmium-exposure group (P<0.01).Compared with control group, mitochondrial ROS levels of hematopoietic stem cells significantly increased in cadmium-exposure group and was dose-related(P<0.05,P<0.01). Besides, mitochondrial membrane potential of hematopoietic stem cells decreased in cadmium -exposure group compared with control group and was dose-related(P<0.05,P<0.01).Conclusion Cadmium exposure can lead to dose-related mitochondrial dysfunction of hematopoietic stem cells via oxidative damage in Kunming mice .