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目的:比较新疆紫草根及茎残基部分成分及含量。方法:采用薄层色谱法及HPLC指纹图谱鉴别新疆紫草成分;紫外-可见分光光度法测定新疆紫草中羟基萘醌总色素含量;HPLC测定β-β’-二甲基丙烯酰阿卡宁和紫草素的含量总和。结果:10批药材根与茎残基部分薄层斑点数目、形状一致,根的斑点大、色泽深;指纹图谱中两者指纹特征相同,相同保留时间下,根的峰面积更大;10批药材中根的羟基萘醌总色素含量平均为3.631%,茎残基平均为1.516%,紫草素与β-β’-二甲基丙烯酰阿卡宁的含量总和的平均值在根及茎残基中分别为0.896%、0.309%。结论:新疆紫草根与茎残基部分所含成分基本一致,但根中羟基萘醌总色素含量、β-β’-二甲基丙烯酰阿卡宁与紫草素总含量均高于茎残基部分两倍以上。
Objective: To compare the components and contents of root and stem residues of Lithospermum. Methods: Lithospermum constituents were identified by TLC and HPLC fingerprinting. The total content of hydroxynaphthoquinone in Xinjiang Lithospermum was determined by UV-Vis spectrophotometry. The content of β-β’-dimethylacryloyl-akainin And shikonin content of the sum. Results: The numbers of spots and roots in the roots and stems of 10 batches of medicinal plants were the same with the same shape. The spots of the roots were large and the color was deep. The fingerprints of the two fingerprints were the same in the fingerprints. The peak area of the root was greater under the same retention time. The average content of hydroxynaphthoquinone in the roots of medicinal herbs was 3.631% on average, and the average of stem residues was 1.516%. The average of the contents of shikonin and β-β’-dimethylacryloracan at the roots and stems Base were 0.896%, 0.309%. CONCLUSION: The components of roots and stems of Lithospermum indicum are basically the same, but the content of total hydroxynaphthoquinones and the total content of β-β’-dimethylacryloracan and shikonin in roots are higher than those of stems Base part more than twice.