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目的：探讨ＢＨＲＦ１ 反义寡核苷酸对表达ＢＨＲＦ１ 的鼻咽癌细胞株ＣＮＥ２ 生存能力的影响。方法：检测反义阻断ＢＨＲＦ１ 表达后ＢＨＲＦ１－ＣＮＥ２细胞在喜树碱作用下增殖能力和凋亡指数的变化。结果：在喜树碱作用下，应用ＢＨＲＦ１ 反义寡核苷酸组的癌细胞较对照组的增殖率下降，凋亡率增高。结论：反义阻断ＢＨＲＦ１的表达可降低ＣＮＥ２细胞的生存能力，这在鼻咽癌的治疗中可能具有一定的价值
Objective: To investigate the effect of BHRF1 antisense oligonucleotide on the viability of CNE2 cell line expressing nasopharyngeal carcinoma cell line BHRF1. Methods: The proliferation and apoptosis index of BHRF1-CNE2 cells treated with anti-sense BHRF1 were detected by MTT assay. Results: Under the action of camptothecin, the proliferation rate of cancer cells using BHRF1 antisense oligodeoxynucleotide group decreased compared with the control group, and the apoptosis rate increased. CONCLUSION: Anti-sense blockade of BHRF1 expression can reduce the viability of CNE2 cells, which may be of value in the treatment of nasopharyngeal carcinoma