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目的对腹泻住院患儿肠道腺病毒的核酸进行检测分析,以了解武威地区腹泻患儿肠道腺病毒的感染情况。方法收集2012年-2014年900例临床确诊病毒性腹泻患儿的粪便标本和临床资料,用逆转录PCR(RT-PCR)法检测腺病毒。结果 900例患儿中,腺病毒感染140例(15.56%),2012年-2014年每年度腺病毒阳性检出率分别为10.33%、19.00%、17.33%,差异有统计学意义(χ~2=9.66,P<0.05)。男性患儿腺病毒检出率较女性患儿高,腺病毒检出率与患儿年龄和发病时间有关。腺病毒检出患儿和腺病毒非检出患儿的日均腹泻和呕吐次数比较,差异有统计学意义(P<0.05),其他临床症状如发热程度、脱水等症状均相似。结论肠道腺病毒是感染武威地区婴幼儿腹泻的主要病原体之一,建立完善的腺病毒流行监测系统是当前预防与控制婴幼儿腹泻的重要环节。
Objective To detect and analyze the nucleic acid of intestinal adenovirus in hospitalized children with diarrhea in order to understand the infection status of intestinal adenovirus in children with diarrhea in Wuwei area. Methods Stool samples and clinical data of 900 clinically diagnosed children with viral diarrhea from 2012 to 2014 were collected. The adenovirus was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results Of the 900 children, 140 (15.56%) were adenovirus-infected and the annual positive rate of adenovirus was 10.33%, 19.00% and 17.33% respectively from 2012 to 2014 (χ ~ 2 = 9.66, P <0.05). Male patients with adenovirus detection rate higher than women with children, adenovirus detection rate and the age of children and the onset time. The average number of diarrhea and vomiting in children with and without adenovirus detected by adenovirus was statistically significant (P <0.05). Other clinical symptoms such as fever, dehydration and other symptoms were similar. Conclusions Intestinal adenovirus is one of the major pathogens in infantile diarrhea in Wuwei area. Establishing a perfect surveillance system for adenovirus is an important part of prevention and control of diarrhea in infants and young children.