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目的研究细胞凋亡相关基因Ｃ－ｆｏｓ及ｂｅｌ－２表达在缺血性脑血管病中的作用并探讨蝮蛇抗栓酶、尼莫地平作用的分子学机理。方法应用免疫组化法观察脑缺血大鼠额、顶叶Ｃ－ｆｏｓ及ｂｅｌ－２的阳性细胞数。并应用蝮蛇抗栓酶、尼莫地平以观察其影响。结果各组大鼠脑额、顶叶均有Ｃ－ｆｏｓ及ｂｅｌ－２的阳性神经元。额、顶叶Ｃ－ｆｏｓ明显增强（Ｐ＜０．０１），ｂｅｌ－２表达似乎增强，但无统计学意义。蝮蛇抗栓酶对Ｃ－ｆｏｓ有较强的抑制作用，对ｂｅｌ－２作用不明显。尼莫地平对两者的影响均不明显。结论Ｃ－ｆｏｓ表达和ｂｅｌ－２表达与缺血性神经元功能损害关系密切，提示对脑缺血患者应用抑制Ｃ－ｆｏｓ表达和增强ｂｅｌ－２表达的药物非常必要。
Objective To study the role of apoptosis-related genes C-fos and bel-2 in ischemic cerebrovascular disease and to explore the molecular mechanism of the antithrombin enzyme and nimodipine. Methods The positive cells of C-fos and bel-2 in frontal and parietal lobe of rats with cerebral ischemia were observed by immunohistochemistry. And the application of viper antithrombotic enzyme, nimodipine to observe the impact. Results The positive neurons of C-fos and bel-2 in the frontal and frontal lobes of rats in each group were positive. The amount of C-fos in the frontal and parietal lobes was significantly increased (P <0.01), and the expression of bel-2 seemed to be enhanced but not statistically significant. Agkistrodon antithrombin has a strong inhibitory effect on C-fos and has no obvious effect on bel-2. The effect of nimodipine on both is not obvious. Conclusions The expression of C-fos and the expression of bel-2 are closely related to the functional impairment of ischemic neurons, suggesting that it is necessary to use drugs that inhibit the expression of C-fos and enhance the expression of bel-2 in patients with cerebral ischemia.