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目的 研究核因子－κＢ（ＮＦ－κＢ） 在百日咳菌液致大鼠感染性脑水肿模型中的活性变化及热休克反应对其影响。方法 动物随机分为：①正常对照组（ＮＳ） ，②感染性脑水肿组（ＰＢ），③热休克处理组（ＨＳ＋ ＰＢ）。取左大脑半球全层脑组织分别测定脑组织含水量，干脑组织钠、钾离子含量；采用电泳迁移率改变法检测各组神经细胞核内ＮＦ－κＢＤＮＡ－ 蛋白结合物活性的变化并做密度扫描定量分析。结果 ＨＳ＋ ＰＢ各组脑组织含水量明显低于ＰＢ组（ Ｐ＜０ ．０１），ＨＳ＋ ＰＢ 各组Ｎａ＋ 含量明显低于ＰＢ组（Ｐ＜ ０．０１ 或Ｐ＜ ０．０５） ，Ｋ＋ 含量除２４ ｈ 之外，均明显高于ＰＢ组（ Ｐ＜０ ．０１） 。与正常对照组比较，２ ｈ 感染性脑水肿组ＮＦ－κＢ活性开始增加，并逐渐增高，以２４ ｈ 为最明显。热休克处理组各时相ＮＦ－ κＢ 活性均有不同程度减低。结论 大鼠感染性脑水肿时ＮＦ－ κＢ活性增加，热休克反应对大鼠感染性脑水肿的保护作用可能与抑制ＮＦ－κＢ的活性有关。
Objective To study the changes of nuclear factor-κB (NF-κB) activity in pertussis bacilli-induced infectious brain edema model and the effect of heat shock response. Methods The animals were randomly divided into: ① normal control group (NS), ② infectious brain edema group (PB), ③ heat shock treatment group (HS + PB). Whole brain tissues of the left hemisphere were taken to measure the water content of brain tissue and the contents of sodium and potassium in the brain tissue respectively. The changes of NF-κB DNA-protein conjugate activity in each group were detected by density shift electrophoresis Quantitative analysis. Results The water content of brain tissue in HS + PB group was significantly lower than that in PB group (P <0.01). The content of Na + in HS + PB group was significantly lower than that in PB group (P <0.01 or P <0.05) 24 h, were significantly higher than the PB group (P <0 .01). Compared with the normal control group, the activity of NF-κB in 2-hour infectious brain edema began to increase and gradually increased, with 24 h being the most obvious. Heat shock treatment group at each phase of NF-κB activity were reduced to varying degrees. Conclusions The activity of NF-κB increases in rats with infectious brain edema, and the protective effect of heat shock reaction on encephalopathy in rats may be related to the inhibition of the activity of NF-κB.