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目的研究龙胆提取物中龙胆苦苷的含量测定和鉴别方法,制定提取物的质量标准。方法采用薄层层析法和高效液相法,对提取物中的龙胆苦苷进行定性鉴别和含量测定。结果通过方法学系统考察和样品的含量测定,建立了龙胆提取物中龙胆苦苷的含量测定方法,测得龙胆苦苷的线性范围为15.84~1014μg·mL-1,回归方程Y=159014.83+20306106.76X,r=0.9997,平均回收率为96.57%,RSD为1.78%。结论本实验建立的鉴别和含量测定方法具有专属性强,重现性好,无明显干扰等优点,是控制本品内在标准较理想的方法。
Objective To study the determination and identification of gentiopicrin in gentian extract and establish the quality standard of the extract. Methods The gentiopicrin in extracts was identified and determined by thin layer chromatography and high performance liquid chromatography. RESULTS: The determination method of gentiopicroside in gentian extract was established by methodological study and determination of samples. The linear range of gentiopicrin was 15.84-1014 μg·mL-1. The regression equation Y= 159014.83+20306106.76X, r=0.9997, the average recovery rate was 96.57%, and the RSD was 1.78%. Conclusion The method of identification and content determination established in this experiment has the advantages of strong specificity, good reproducibility, no obvious interference, etc. It is an ideal method to control the internal standard of the product.