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目的 探讨人乳头瘤病毒 (humanpapillomavirus,HPV) 16及其血清抗体与胃癌发生的关系。方法 采用多聚酶链反应 (polymerasechainreaction ,PCR)技术检测 42例新鲜胃癌组织标本及其癌旁正常胃粘膜标本中的HPVDNA ;并采用重组杆状病毒 昆虫细胞系统制备的HPV16病毒样颗粒 (viruslikeparticles,VLPs) ,通过酶联免疫吸附试验 (enzymelinkedimmunosorbentassay ,ELISA)方法检测上述胃癌患者及 46例健康对照者的血清抗体。结果 胃癌及癌旁正常胃粘膜组织中HPV16DNA的检出率分别为 2 6 % (11/ 42 )和 0 (0 / 42 ) ,差异有显著性 (P <0 0 1) ;胃癌组及健康对照组HPV16VLPs血清抗体A均值分别为 0 0 6 9± 0 0 2 6和 0 0 36± 0 0 2 7,两组差异有显著性 (P <0 0 5 ) ;HPV阳性和阴性胃癌患者HPV16抗体A均值分别为 0 0 81± 0 0 2 7和 0 0 42± 0 0 16 ,两者差异有显著性(P <0 0 1)。结论 HPV16感染可能与胃癌的发生有关。HPV16血清抗体检测可用作HPV感染的血清学诊断指标和用于HPV相关性肿瘤的免疫学研究。
Objective To investigate the relationship between human papillomavirus (HPV) 16 and serum antibodies and the occurrence of gastric cancer. Methods Polymerase chain reaction (PCR) technique was used to detect HPV DNA in 42 specimens of fresh gastric cancer tissues and normal gastric mucosa around the tumors. HPV16 viruslike particles (VLPs) were prepared using recombinant baculovirus insect cell system. Serum antibodies were detected by enzyme linked immunosorbent assay (ELISA) in these gastric cancer patients and 46 healthy controls. Results The detection rates of HPV16 DNA in gastric cancer and adjacent normal gastric mucosa were 2 6% (11/42) and 0 (0/42), respectively. The difference was statistically significant (P < 0 01). Gastric cancer group and healthy controls The serum A antibody of HPV16VLPs was 0 0 69 + 0 0 2 6 and 0 0 36 ± 0 0 2 7, respectively. There was a significant difference between the two groups (P <0 05). HPV16 antibody A in HPV positive and negative gastric cancer patients. The mean values were 0 0 81 ± 0 0 2 7 and 0 0 42 ± 0 0 16 , respectively. There was a significant difference between them (P <0 01). Conclusion HPV16 infection may be related to the occurrence of gastric cancer. The HPV16 serum antibody test can be used as a serological diagnostic marker for HPV infection and for immunological studies of HPV-associated tumors.