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目的:观察痛痹颗粒对大鼠自由基代谢及血液流变性的影响,探讨痛痹颗粒治疗骨关节炎的可能机制。方法:采用16月龄SD大鼠,连续给予痛痹颗粒45天,末次给药后取血清测SOD、MDA含量。采用皮下注入肾上腺素加冰水复制血瘀模型,测定全血黏度、血浆黏度、红细胞聚集指数,刚性指数等指标。结果:痛痹颗粒明显提高老龄大鼠血清中SOD活性,降低MDA含量,与老龄组比较具有显著性差异(P<0.01)。对血瘀大鼠可明显降低全血黏度值、血浆黏度值、红细胞聚集指数和红细胞刚性指数,与模型组比较具有显著性差异(P<0.01)。结论:痛痹颗粒治疗骨关节炎的作用机制可能与改善机体血液流变性,清除氧自由基和抗衰老作用有关。
Objective: To observe the effects of Tongbi granule on free radical metabolism and hemorrheology in rats, and explore the possible mechanism of Tongbi Granule in treating osteoarthritis. Methods: 16-month-old SD rats were used to continuously give Tongbi granule for 45 days. Serum SOD and MDA levels were measured after the last administration. Subcutaneous injection of epinephrine plus ice water to replicate blood stasis model, determination of whole blood viscosity, plasma viscosity, erythrocyte aggregation index, rigidity index and other indicators. Results: Tongbi granule could significantly increase the activity of SOD in the serum of aged rats and decrease the content of MDA, which was significantly different from the old group (P <0.01). Blood stasis rats can significantly reduce the whole blood viscosity, plasma viscosity, erythrocyte aggregation index and erythrocyte rigidity index, compared with the model group had significant difference (P <0.01). Conclusion: The mechanism of Tongbi Granule in treating osteoarthritis may be related to the improvement of blood rheology, scavenging oxygen free radicals and anti-aging effects.